[Cloning and expression of SjMRLC and analysis of its stage-specific transcription].


OBJECTIVE To clone and express Schistosoma japonicum myosin regulatory light chain (SjMRLC) in prokaryotic expression system and analyze its stage-specific transcription. METHODS The recombinant plasmid was constructed with pGEX 4T-3 vector and expressed in BL21. The recombinant SjMRLC was identified through Western blotting assay. Total RNA from… (More)