[Cloning and expression of Mycobacterium bovis secreted protein MPB51 in Escherichia coli].


The gene encoding MPB51 was amplified from M. bovis Valleel11 chromosomal DNA using PCR technique, and the PCR product was approximately 800 bp DNA segment. Using T-A cloning technique, the PCR product was cloned into pGEM-T vector and cloning plasmid pGEM-T-51 was thus constructed successfully. pGEM-T-51 and pET28a( + ) were digested by BamH I and EcoR I… (More)


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