[Cloning and expression of F18 fimbrial operon gene clusters from enterotoxigenic Escherichia coli and their bioactivity].

Abstract

The fed operon gene clusters with each size of 5.6kb, encoding the F18ab or F18ac fimbriae, was amplified respectively by high fidelity PCR using the genomic DNA templates from F18 fimbriae E. coli strains 107/86 or 2134P. The PCR products with the restriction enzyme sites at each end were digested and then cloned into the vector pET-22b (+), the… (More)

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Cite this paper

@article{Zhang2007CloningAE, title={[Cloning and expression of F18 fimbrial operon gene clusters from enterotoxigenic Escherichia coli and their bioactivity].}, author={Jian-Jun Zhang and Guo-Qiang Zhu}, journal={Wei sheng wu xue bao = Acta microbiologica Sinica}, year={2007}, volume={47 5}, pages={790-4} }