[Cloning and characterization of the promoter region of Musca domestica yolk protein-1 gene].

@article{Xia2004CloningAC,
  title={[Cloning and characterization of the promoter region of Musca domestica yolk protein-1 gene].},
  author={Ping-An Xia and W Liu and Yu Jiang and Shao-guang Sun and Shu-Yan Yang and Ji-gui Wang},
  journal={Yi chuan xue bao = Acta genetica Sinica},
  year={2004},
  volume={31 7},
  pages={688-94}
}
A partial Musca domestica genomic library was constructed. It was consisted of 1.2 x 10(5) recombinants with insert length ranging from 10 kb to 23 kb(15 kb average). High molecular weight genomic DNA with more than 50 kb size was extracted from the larva hatched 36 h and digested with unfrequently cutting restriction enzyme Bcl I. DNA fragments of 10 approximately 23 kb were recovered by agarose gel electrophoresis and ligated with EMBL3 BamH I Arms CIPase treated. Then the products of… CONTINUE READING