[Cloning and bioinformatics analysis of cycloartenol synthase (HcCAS1) gene in Huperzia carinata].


OBJECTIVE To clone and sequence the open reading frame of cycloartenol synthase (CAS) from Huperzia carinata. METHOD After searching the transcriptome dataset of H. carinata, one unique sequence containing oxide squalene cyclases domain was discovered. The primers were designed according to the cDNA sequence of CAS from the dataset. And then, the open… (More)


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