[Cloning, expression analysis and RNA interference of FGF5 gene in sheep].

@article{Liu2011CloningEA,
  title={[Cloning, expression analysis and RNA interference of FGF5 gene in sheep].},
  author={Wu-Xian Liu and Bin Jia and Guo-qing Shi and Jian-gong Ren and Ka Liu and Run-lin Z Ma},
  journal={Yi chuan = Hereditas},
  year={2011},
  volume={33 9},
  pages={982-8}
}
The cDNA of fibroblast growth factor 5 (FGF5) gene in sheep was cloned, and the nucleotides sequence homology of FGF5 was compared with other six mammal. In addition, the expression of FGF5 in different tissues was analysed. Gene FGF5 was then recombined into prokaryotic expression vector (pGEX-4T-2) and RNA interference vector (pSilencer 5.1 H1) to study its expression in fibroblast cell lines. Results showed that the open reading frame (ORF) of cDNA in sheep consisted of 813 nucleotide acids… CONTINUE READING