[Chromogranin A gene antisense transgenic mice and the tissue specific promoter of chromogranin A gene].

Abstract

The vector pGAS1C-lacZ was constructed which contains cga promoter-lacZ and cga promoter-cga antisense cDNA two major parts. The pGAS1C-lacZ was integrated into genome of PC-12 cells and lacZ gene expressed in PC-12 cells. We cut the pGAS1C-lacZ with Xba I and Sal I to get rid of the framework of plasmid pGAS1C-lacZ, then microinjected the other part which… (More)

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