[Characterization of DNAse from rat brain].

@article{Ivanov1982CharacterizationOD,
  title={[Characterization of DNAse from rat brain].},
  author={Vadim A. Ivanov and O N Terpilovskaia and T M Tret'iak and Ga{\"i}ina Smirnova},
  journal={Biokhimiia},
  year={1982},
  volume={47 3},
  pages={398-404}
}
Using gel filtration and ion exchange chromatography, a DNAse specific to a single-stranded DNA was isolated from the soluble fraction of rat brain nuclear proteins. The nuclear nuclease corresponds to alkaline DNAse obtained earlier from the intact brain. The enzyme is a Mg2+- or Mn2+-dependent exodeoxyribonuclease and hydrolyzes at the same rates the ss-DNAse from rat brain, calf thymus, E. coli and poly (dA). The major products of hydrolysis are nucleoside-5'-monophosphates. A possible role… CONTINUE READING

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