• Biology, Medicine
  • Published in
    Zhongguo xiu fu chong jian…
    2007

[Characteristics of osteoblastic differentiation in mesenchymal stem cells from porcine bone marrow in vitro].

@article{Zou2007CharacteristicsOO,
  title={[Characteristics of osteoblastic differentiation in mesenchymal stem cells from porcine bone marrow in vitro].},
  author={Lijin Zou and Nonghua Lv and Wenzhou Feng},
  journal={Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery},
  year={2007},
  volume={21 11},
  pages={
          1222-7
        }
}
OBJECTIVE To observe the characteristics and related gene expression of osteoblastic differentiation in porcine bone marrow mesenchymal stem cells (MSCs) during. METHODS Bone marrow from 6 landrace pigs, 3-month-old about 50 kg, was aspirated from the medullary cavity of the proximal tibia. The MSCs were isolated, and purified by Ficoll density gradient centrifugation combined with adherent culture method. The MSCs from passage 1 were cultivated in DMEM with 1 x 10(-8) mmol/L dexamethasone… CONTINUE READING