[Characteristics of isolating influenza virus hemagglutinin using purified bromelin].

@article{Mukazhanova1981CharacteristicsOI,
  title={[Characteristics of isolating influenza virus hemagglutinin using purified bromelin].},
  author={G N Mukazhanova and M. S. Siniakov and Igor G. Kharitonenkov},
  journal={Voprosy virusologii},
  year={1981},
  volume={3},
  pages={
          275-80
        }
}
Gel filtration and ion-exchange chromatography were used to isolate a fraction with the highest proteolytic activity and virtually lacking glycosidases from a commercial bromeline preparation ("Serva", grade B) showing, in addition to proteolytic, also glycosidase activity. The utilization of purified bromeline allows isolation of hemagglutinin without residual neuraminidase activity from influenza virus of various serotypes (H1, H1, and H3). The results are discussed on the basis of an… CONTINUE READING

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