[Baculovirus expression of two human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus].

@article{Cao2004BaculovirusEO,
  title={[Baculovirus expression of two human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus].},
  author={Jing-yuan Cao and Mi-fang Liang and Qing-ling Meng and Xiaofang Wang and Yonggang Xu and Ke-qian Guo and Mei-yun Zhan and Sheng-li Bi and De-xin Li},
  journal={Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology},
  year={2004},
  volume={18 1},
  pages={20-3}
}
OBJECTIVE To develop human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus (HAV) by baculovirus expression system. METHODS The heavy and light chain genes of two human-derived neutralizing Fab antibodies to HAV were cloned into baculovirus expression vector Pac-kappa-Fc and Pac-L-Fc, and further expressed in insect cells as IgG antibodies. The IgG products were purified and well characterized. RESULTS The baculovirus expressed McAb HAFc16 fully retained the… CONTINUE READING