[Arsenic trioxide induced p15INK4B gene expression in myelodysplastic syndrome cell line MUTZ-1].

@article{Tong2002ArsenicTI,
  title={[Arsenic trioxide induced p15INK4B gene expression in myelodysplastic syndrome cell line MUTZ-1].},
  author={Hongyan Tong and Maofang Lin},
  journal={Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi},
  year={2002},
  volume={23 12},
  pages={638-41}
}
OBJECTIVE To investigate the mechanisms of arsenic trioxide (As(2)O(3)) induced demethylation. METHOD Methylation of p15INK4B gene in MUTZ-1 cell was detected by PCR using a methylation specific primer (MSP), the expression of P15, DNA methyltransferase (DNMT) 1, DNMT3A and DNMT3B gene by RT-PCR, the As(2)O(3) induced growth inhibition of MUTZ-1 cell by MTT method. RESULTS P15 gene failed to express in MUTZ-1 cells after methylation. The expression was recovered after the cells exposed to… CONTINUE READING