[Application of fluorescent probe spectrum analysis for the investigation of the structural state of sorbent. Heterogeneous binding sites].


A method is proposed for analyzing the fluorescence spectra of 1-anilinonaphthalene-8-sulphonate (ANS) and N-phenyl-1-naphthylamine (NPN) in the systems with nonuniform binding regions. Capacities of the method are demonstrated by analysing the fluorescence spectra of probes sorbed on bovine albumin molecules. It has been shown that there are two binding… (More)