[Application of PCR for the detection of mutations in the genes coding for beta-lactamases].

@article{Gallego1993ApplicationOP,
  title={[Application of PCR for the detection of mutations in the genes coding for beta-lactamases].},
  author={Luc{\'i}a Gallego and Miren Basaras and Roberto Alonso and L. Sarr{\'i}a and Ram{\'o}n Cisterna},
  journal={Enfermedades infecciosas y microbiologia clinica},
  year={1993},
  volume={11 2},
  pages={80-3}
}
BACKGROUND The purpose of this study was to develop a satisfactory technique to detect punctual mutations in blaTEM genes. METHODS The strains [E. coli HB 101 pBR322 (TEM-1), E. coli J62 RP4 (TEM-2)] were submitted to PCR with primers PL1 and PL2 which amplify the genetic region susceptible of punctual mutations. Then, we developed Southern blot and… CONTINUE READING