[Application of Cre-loxP(*) system in constructing the markerless double-gene-deletion strain in Streptococcus mutans].


OBJECTIVE To construct double gene deletions at the htrA and clpP loci on the chromosome of Streptococcus mutans (Sm) and to remove the antibiotic resistance markers with the Cre-loxP(*) site-specific recombination system. METHODS The htrA gene was cloned into the pGEM-T-Easy TA cloning vector and then inactivated via the insertion of a kanamycin… (More)


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