• Medicine, Chemistry
  • Published in
    Zhongguo Zhong yao za zhi…

[Analysis of dencichine by HPLC with pre-column derivatization].

  title={[Analysis of dencichine by HPLC with pre-column derivatization].},
  author={Jing Zhu and S Liu and Chun-mei Fu and Zhang-wan Li},
  journal={Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica},
  volume={31 22},
OBJECTIVE To establish a reversed-phase high performance liquid chromatorgraphy (RP-HPLC) method for detecting the dencichine in Panax notoginseng extracts and drug preparations. METHOD Dencichine was extracted with the borate buffer (pH 9. 18) and the clear supernatant was used for the derivatization. Pre-column derivatization was performed using 9-fluorenylmethyl chloroformate (FMOC) to form derivatives. The mobile phase consisted of methanol and 0. 05 mol x L( -1) NaH2 PO4 (48: 52) (pH… CONTINUE READING