[Amino oxyadsorbents. New type of adsorbents for affinity chromatography: purification of tRNA-methylases from rat nephron on aminooxybutylcellulose with immobilized tRNA].

@article{Nedospasov1978AminoON,
  title={[Amino oxyadsorbents. New type of adsorbents for affinity chromatography: purification of tRNA-methylases from rat nephron on aminooxybutylcellulose with immobilized tRNA].},
  author={Andrei A Nedospasov and Radii M Khomutov and A S Gambarian and I. A. Morozov and T. V. Venkstern},
  journal={Molekuliarnaia biologiia},
  year={1978},
  volume={12 5},
  pages={1104-11}
}
A new type of sorbents for affinity chromatography is suggested and used to purify tRNA methylases. tRNA was immobilized on aminooxybutylcellulose via the oxidized 3'-end. In order to bind other enzymes specific for nucleic acids in general, e. g. nucleases, and to achieve a higher degree of purification the crude enzyme preparation was treated with rRNA immobilized on aminooxybutycellulose. The sequential application of two sorbents mentioned allows to get an approximately two hundred fold… CONTINUE READING