[Advanced oxidation protein products induce reactive oxygen species production in endothelial cells].

@article{Yuan2004AdvancedOP,
  title={[Advanced oxidation protein products induce reactive oxygen species production in endothelial cells].},
  author={Fang Yuan and Shang-xi Liu and Jian-wei Tian},
  journal={Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA},
  year={2004},
  volume={24 12},
  pages={1350-2}
}
OBJECTIVE To investigate the effect of advanced oxidation protein products (AOPP) on production of reactive oxygen species (ROS) in endothelial cells. METHODS Human umbilical vein endothelial cell line ECV304 were stimulated with AOPP-modified bovine serum albumin (AOPP-BSA) prepared by oxidation of BSA with HOCl in vitro. ROS production in the cells was evaluated by kinetic measurement of dichlorofluoroscein (DCF) fluorescence produced by oxidation of an oxidant-sensitive dye 2,7… CONTINUE READING