Corpus ID: 39133316

[A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies].

  title={[A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies].},
  author={H. Schlegel and H. Kaltwasser and G. Gottschalk},
  journal={Archiv fur Mikrobiologie},
In Vitro Analogues CoA-Thioester Formation of Succinate Conversion of Malate to Malyl-CoA and Coenzyme A ( Succinate-CoA ) Ligases : Novel Characteristics of Succinate
Published Ahead of Print 18 October 2013. 10.1128/AEM.03075-13. 2014, 80(1):166. DOI: Appl. Environ. Microbiol. Wübbeler and Alexander Steinbüchel Catherine-Louise Schepers, Elvira Vogel, Jan HendrikExpand
Novel Characteristics of Succinate Coenzyme A (Succinate-CoA) Ligases: Conversion of Malate to Malyl-CoA and CoA-Thioester Formation of Succinate Analogues In Vitro
Liquid chromatography/electrospray ionization-mass spectrometry analyses proved the ability of SucCD enzymes to form CoA-thioesters of adipate, glutarate, and fumarate and demonstrated that the activation of 3SP is not a unique characteristic of the SucCD from A. mimigardefordensis DPN7T. Expand
Novel Reaction of Succinyl Coenzyme A (Succinyl-CoA) Synthetase: Activation of 3-Sulfinopropionate to 3-Sulfinopropionyl-CoA in Advenella mimigardefordensis Strain DPN7T during Degradation of 3,3′-Dithiodipropionic Acid
It is concluded that SucCD(Am) is physiologically associated with the citric acid cycle but is mandatory for the catabolic pathway of DTDP and its degradation intermediate 3SP. Expand
In Vivo Enzyme Immobilization by Use of Engineered Polyhydroxyalkanoate Synthase
It is demonstrated that engineered polyhydroxyalkanoate (PHA) synthases can be employed as molecular tools to covalently immobilize enzymes at the PHA granule surface, suggesting an application in recycling of biocatalysts, such as in fine-chemical production. Expand
Carbohydrate uptake in Advenella mimigardefordensis strain DPN7T is mediated by periplasmic sugar oxidation and a TRAP‐transport system
An uptake assay with radiolabeled [14C(U)]‐d‐glucose clearly showed that the deletion of dctPAm, dctQ and dctM disabled the uptake of this aldoses in cells of either mutant strain, proving oxidation of the molecules in the periplasm, prior to transport. Expand
Exploiting mixtures of H2, CO2, and O2 for improved production of methacrylate precursor 2-hydroxyisobutyric acid by engineered Cupriavidus necator strains
Analyzing the inhibition of growth and product synthesis due to substrate availability and product accumulation revealed a strong influence of 2-HIBA, when cells were cultivated at high titers, implying that at the time the autotrophic synthesis route is superior to thus far established heterotrophic routes for production of 2 -HIBA with C. necator. Expand
Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate
To the first report where PE is used as a starting material for production of a biodegradable polymer, the addition of biosurfactant allowed for greater bacterial growth and PHA accumulation of the tested strains. Expand
Succinyl-CoA:3-Sulfinopropionate CoA-Transferase from Variovorax paradoxus Strain TBEA6, a Novel Member of the Class III Coenzyme A (CoA)-Transferase Family
Analysis of the translation product clearly allocated Act(TBEA6) to the succinyl-CoA:3-sulfinopropionate coenzyme A (CoA)-transferase family and unequivocally verified the conversion of 3SP to 3SP- coA. Expand
The role of AlgK in alginate biosynthesis by Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements of the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand
It is demonstrated that AlgK is essential for polymerisation and is required for the stability of components involved in polymerisation, translocation, and secretion (AlgE), and it is shown that Al gK interacts with periplasmic AlgX but not with inner membrane Al g44 or outer membrane AlgE. Expand
A new monocupin quercetinase of Streptomyces sp. FLA: identification and heterologous expression of the queD gene and activity of the recombinant enzyme towards different flavonols
The gene queD encoding quercetinase of Streptomyces sp. FLA, a soil isolate related to S. eurythermusT, was identified. Quercetinases catalyze the 2,4-dioxygenolytic cleavage ofExpand