[A simple method for assessment of RNA integrity in laser capture microdissection samples].

@article{Tian2008ASM,
  title={[A simple method for assessment of RNA integrity in laser capture microdissection samples].},
  author={Ying-fang Tian and Zhao-ming Wei and Xinlin Linda Chen and Fen Qiu and Xin-li Xiao and Qian-yan Kang and Bofeng Zhu and Yu-mei Tian and Jun-feng Zhang and Yong Liu},
  journal={Nan fang yi ke da xue xue bao = Journal of Southern Medical University},
  year={2008},
  volume={28 10},
  pages={1782-5}
}
OBJECTIVE To develop a simple method for assessment of RNA integrity in laser capture microdissection (LCM) samples. METHODS The total RNA were isolated from the LCM samples and the sections before and after microdissection and examined by agarose gel electrophoresis. Real-time PCR was employed to assess the RNA from LCM samples, and the quantity of RNA was theoretically estimated according to the average total RNA product in mammalian cells (10 ng/1000 cells). RESULTS When the total RNA… CONTINUE READING