[83A] Determination of aspartic acid and asparagine

  title={[83A] Determination of aspartic acid and asparagine},
  author={Samuel P. Bessman},
Publisher Summary Aspartic acid, asparagine, glutamic acid, and glutamine can be determined in the same specimen by making use of two principles. The dicarboxylic amino acids are adsorbed on acid-treated alumina and can be eluted separately, glutamic acid by acid elution, and aspartic acid by alkaline elution. Neither glutamine nor asparagine is adsorbed on the alumina. The solution from which the dicarboxylic acids have been removed is then subjected to acid hydrolysis to convert glutamine and… CONTINUE READING