[5] Liposomes as carriers for the transfer and expression of nucleic acids into higher plant protoplasts

@inproceedings{Caboche19875LA,
  title={[5] Liposomes as carriers for the transfer and expression of nucleic acids into higher plant protoplasts},
  author={Michel Caboche and Paul F. Lurquin},
  year={1987}
}
Publisher Summary This chapter presents an alternative method based on the encapsulation of nucleic acids into large unilamellar liposomes. This technique has proved to yield high viral RNA-mediated transfection values, and in one case, is shown to be the only one allowing successful transfection of protoplasts with potyviral RNA. In addition, encapsulation of recombinant DNA into such liposomes followed by the interaction with protoplasts is shown to enable the recovery of regenerated tobacco… CONTINUE READING

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