[3H]WIN 35,065–2: A Ligand for Cocaine Receptors in Striatum
@article{Ritz19903HWIN3A, title={[3H]WIN 35,065–2: A Ligand for Cocaine Receptors in Striatum}, author={Mary C. Ritz and John W. Boja and Dimitri E. Grigoriadis and Robert C. Zaczek and F. Ivy Carroll and Amanda H Lewis and Michael J. Kuhar}, journal={Journal of Neurochemistry}, year={1990}, volume={55} }
Abstract: [3H]WIN 35,065–2 binding to striatal membranes was characterized, primarily by centrifugation assay. Like [3H]cocaine, [3H]WIN 35,065–2 binds to both high‐ and low‐affinity sites. [3H]WIN 35,065–2, however, exhibits consistently higher affinities than [3H]cocaine. Saturation experiments indicate a low‐affinity binding site with an apparent KD of ∼ 160 nM and a Bmaxof 135 fmol/mg of tissue. A high‐affinity site has also been identified with an apparent KD of 5.6 nM and a Bmax of 5.2…
39 Citations
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