[3H]WIN 35,065–2: A Ligand for Cocaine Receptors in Striatum

@article{Ritz19903HWIN3A,
  title={[3H]WIN 35,065–2: A Ligand for Cocaine Receptors in Striatum},
  author={Mary C. Ritz and John W. Boja and Dimitri E. Grigoriadis and Robert C. Zaczek and F. Ivy Carroll and Amanda H Lewis and Michael J. Kuhar},
  journal={Journal of Neurochemistry},
  year={1990},
  volume={55}
}
Abstract: [3H]WIN 35,065–2 binding to striatal membranes was characterized, primarily by centrifugation assay. Like [3H]cocaine, [3H]WIN 35,065–2 binds to both high‐ and low‐affinity sites. [3H]WIN 35,065–2, however, exhibits consistently higher affinities than [3H]cocaine. Saturation experiments indicate a low‐affinity binding site with an apparent KD of ∼ 160 nM and a Bmaxof 135 fmol/mg of tissue. A high‐affinity site has also been identified with an apparent KD of 5.6 nM and a Bmax of 5.2… 

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  • J.M.M. WilsonJN Nobrega S. Kish
  • Biology, Psychology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1994
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