[3H]Threo‐(±)‐Methylphenidate Binding to 3,4‐Dihydroxyphenylethylamine Uptake Sites in Corpus Striatum: Correlation with the Stimulant Properties of Ritalinic Acid Esters

@article{Schweri19853HThreoMethylphenidateBT,
  title={[3H]Threo‐(±)‐Methylphenidate Binding to 3,4‐Dihydroxyphenylethylamine Uptake Sites in Corpus Striatum: Correlation with the Stimulant Properties of Ritalinic Acid Esters},
  author={Margaret M. Schweri and Phil Skolnick and Michael Francis Rafferty and Kenner C. Rice and Aaron Janowsky and Steven M Paul},
  journal={Journal of Neurochemistry},
  year={1985},
  volume={45}
}
Abstract: Saturable and stereoselective binding sites for [3H]threo‐(±)‐methylphenidate were characterized in rat brain membranes. The highest density of [3H]threo‐(±)‐ methylphenidate binding sites was found in the synapto somal fraction of corpus striatum. Scatchard analysis revealed a single class of noninteracting binding sites with an apparent dissociation constant (KD) of 235 nM and a maximum number of binding sites (Bmax) of 13.4 pmol/mg protein. Saturable, high‐affinity binding of [3H… 

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