[15-Hydroxyprostaglandin dehydrogenase from human placenta, II. Steady state kinetics and influence of prostaglandin F2alpha analogues (author's transl)].

@article{Rckrich197515HydroxyprostaglandinDF,
  title={[15-Hydroxyprostaglandin dehydrogenase from human placenta, II. Steady state kinetics and influence of prostaglandin F2alpha analogues (author's transl)].},
  author={M F R{\"u}ckrich and Albrecht Wendel and Werner Schlegel and Rolf Jackisch and Albrecht Jung},
  journal={Hoppe-Seyler's Zeitschrift fur physiologische Chemie},
  year={1975},
  volume={356 6},
  pages={
          799-809
        }
}
A complete initial rate analysis of the forward reaction catalyzed by 15-hydroxyprostaglandin dehydrogenase from human term placenta was carried out at pH 7.4 (100mM triethanolamine) with the substrates NAD, and the prostaglandins E1, E2 and F2alpha. The limiting Michaelis constants, the dissociation constants, and the limiting maximum velocities for these substrates were calculated by fitting the obtained data by weighted linear regression analysis to the complete rate equation. The product… 
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