[1-13C]Glucose entry in neuronal and astrocytic intermediary metabolism of aged rats A study of the effects of nicergoline treatment by 13C NMR spectroscopy

@article{Miccheli2003113CGlucoseEI,
  title={[1-13C]Glucose entry in neuronal and astrocytic intermediary metabolism of aged rats A study of the effects of nicergoline treatment by 13C NMR spectroscopy},
  author={Alfredo Miccheli and Caterina Puccetti and Giorgio Capuani and Maria Enrica Di Cocco and Luciana Giardino and Laura Calz{\`a} and Angelo Battaglia and Leontino Battistin and Filippo Conti},
  journal={Brain Research},
  year={2003},
  volume={966},
  pages={116-125}
}
Age-related changes in glucose utilization through the TCA cycle were studied using [1-13C]glucose and 13C, 1H NMR spectroscopy on rat brain extracts. Significant increases in lactate levels, as well as in creatine/phosphocreatine ratios (Cr/PCr), and a decrease in N-acetyl-aspartate (NAA) and aspartate levels were observed in aged rat brains as compared to adult animals following glucose administration. The total amount of 13C from [1-13C]glucose incorporated in glutamate, glutamine, aspartate… Expand
Alteration of Interaction Between Astrocytes and Neurons in Different Stages of Diabetes: a Nuclear Magnetic Resonance Study Using [1-13C]Glucose and [2-13C]Acetate
TLDR
The overall metabolism disturbances, changes in specific metabolic pathways, and interaction between astrocytes and neurons during the onset and development of diabetes are demonstrated and contribute to the mechanistic understanding of diabetes pathogenesis and evolution. Expand
Metabolic profiling by 13C-NMR spectroscopy: [1,2-13C2]glucose reveals a heterogeneous metabolism in human leukemia T cells.
TLDR
Results revealed a heterogeneous metabolism in an asynchronous cell population that may be interpreted on the basis of different metabolic phenotypes of subpopulations in relation to different cell cycle phases. Expand
Dietary variation of protein–carbohydrate: Effect on hypothalamic and hippocampal GABA–glutamate in relation to aging
TLDR
The results suggest that an age-associated change in GABA–glutamate metabolism depends on the amount of dietary protein and carbohydrate and also on the brain region. Expand
Long-term Exposure of Variable Dietary Protein-to-Carbohydrate Ratio: Effect on Brain Regional Glutamatergic Activity with Age
TLDR
It may be suggested that long-term consumption of LP–HC and HP–LC diets modulate the brain regional glutamatergic activity reversibly with age. Expand
Longitudinal Quantification of Metabolites and Macromolecules Reveals Age- and Sex-Related Changes in the Healthy Fischer 344 Rat Brain
TLDR
This study reports, for the first time, the simultaneous absolute quantification of brain metabolites and individual macromolecules in aging male and female Fischer 344 rats, measured longitudinally using proton magnetic resonance spectroscopy at 7T. Expand
Longitudinal quantification of metabolites and macromolecules reveals age- and sex-related changes in the healthy Fischer 344 rat brain
TLDR
This study reports, for the first time, the simultaneous absolute quantification of brain metabolites and individual macromolecules in aging male and female Fischer 344 rats, measured longitudinally using proton magnetic resonance spectroscopy at 7 T. Expand
Glucose improvement of memory: a review.
  • C. Messier
  • Psychology, Medicine
  • European journal of pharmacology
  • 2004
TLDR
A small number of studies showed that glucose improvement of memory is associated with poor glucose regulation although this may not be the case for diabetic patients, and results of a few studies suggest that drug treatments that improve glucose regulation also produce cognitive improvement in diabetic patients. Expand
Cerebral Mitochondrial Function and Cognitive Performance during Aging: A Longitudinal Study in NMRI Mice
TLDR
A close connection is demonstrated between the age-related decline of cognitive performance, energy metabolism, and mitochondrial biogenesis during the physiological brain aging process. Expand
Effects of Chronic Photobiomodulation with Transcranial Near-Infrared Laser on Brain Metabolomics of Young and Aged Rats.
TLDR
Chronic transcranial photobiomodulation significantly enhances the metabolic pathways of young rats, particularly for excitatory neurotransmission and oxidative metabolism, and restores the altered metabolic paths of aged rats towards levels found in younger rats, mainly in the cerebral cortex. Expand
Nicergoline, a drug used for age-dependent cognitive impairment, protects cultured neurons against β-amyloid toxicity
TLDR
Experiments carried out in pure cultures of astrocytes showed that a component of neuroprotection was mediated by a mechanism of glial-neuronal interaction, raising the possibility that nicergoline reduces neurodegeneration in the Alzheimer's brain. Expand
...
1
2
...

References

SHOWING 1-10 OF 53 REFERENCES
The entry of [1-13C]glucose into biochemical pathways reveals a complex compartmentation and metabolite trafficking between glia and neurons: a study by 13C-NMR spectroscopy
TLDR
The appearance of the 13C-label in C5 of glutamate and glutamine, C1 of GABA and C2 of lactate, is suggestive of pyruvate, formation from TCA cycle intermediates and provides evidence of metabolite trafficking between astrocytes and neurons. Expand
Aging brain: effect of acetyl-l-carnitine treatment on rat brain energy and phospholipid metabolism. A study by31P and1H NMR spectroscopy
TLDR
Results are suggestive of treatment with ALCAR being responsible for a reduction in brain glycolytic flow and for enhancing the utilization of alternative energy sources, such as lipid substrates or ketone bodies. Expand
In vivo13C NMR measurement of neurotransmitter glutamate cycling, anaplerosis and TCA cycle flux in rat brain during [2‐13C]glucose infusion
TLDR
The findings indicate that anaplerotic glutamine synthesis is coupled to nitrogen removal from the brain (ammonia detoxification) under hyperammonemic conditions. Expand
Acetyl-l-carnitine modulates glucose metabolism and stimulates glycogen synthesis in rat brain
TLDR
The results suggest that acetyl-L-carnitine was able to modulate cerebral glucose utilization and provide new insights on the mechanisms of action of this molecule in the central nervous system. Expand
In Vivo Effects of Adenosine A1 Receptor Agonist and Antagonist on Neuronal and Astrocytic Intermediary Metabolism Studied with Ex Vivo 13C NMR Spectroscopy
TLDR
The findings in the [1,2‐ 13C]acetate and [1‐13C]glucose control, CCPA, and DPCPX groups were complementary, and no adenosine A1 agonist effects arising from cerebral acetate metabolism were detected. Expand
Cerebral Metabolic Compartmentation as Revealed by Nuclear Magnetic Resonance Analysis of D‐[1‐13C]Glucose Metabolism
TLDR
The results of this study support the concept that there are several pools of glutamate, with different metabolic turnover rates, and that glutamine and/or a tricarboxylic acid cycle intermediate is supplied by astrocytes to neurons for replenishing the neurotransmitter pool of GABA. Expand
Age-related changes in the uptake and release of glutamate and aspartate in the mouse brain
TLDR
The decreased uptake capacity together with an increased release of glutamate may contribute to the degenerative changes associated with normal brain ageing and also to the pathogenesis of age-related neurodegenerative disorders. Expand
The effect of age on glucose and energy metabolism in brain cortex of rats.
  • S. Hoyer
  • Chemistry, Medicine
  • Archives of gerontology and geriatrics
  • 1985
TLDR
It is concluded that glucose and energy metabolism may diminish with the process of normal aging, but that the reduction is of only moderate extent. Expand
Cerebral metabolism of acetate and glucose studied by 13C-n.m.r. spectroscopy. A technique for investigating metabolic compartmentation in the brain.
TLDR
The time courses of incorporation of 13C from 13C-labelled glucose or acetate into cerebral amino acids (glutamate, glutamine and 4-aminobutyrate) and lactate were monitored to provide the possibility of distinguishing differential effects of metabolic perturbations on the two pools simultaneously. Expand
Age-Related Changes in Cerebral Oxidative Metabolism
TLDR
Glucose metabolism in the brain is of central significance, and there is increasing evidence that the sum of these metabolic variations develops in a self-propagating manner, following the principle of self-organised criticality. Expand
...
1
2
3
4
5
...