[(35)S]GTPγS binding and opioid tolerance and efficacy in mouse spinal cord.


The present study examined efficacy of a series of opioid agonists and then using chronic in vivo treatment protocols, determined tolerance to opioid agonist stimulated [(35)S]GTPγS (guanosine 5'-O-(3-[(35)S] thio)triphosphate) binding in mouse spinal cord membranes and compared it directly to spinal analgesic tolerance. The [(35)S]GTPγS binding assay was… (More)
DOI: 10.1016/j.pbb.2011.11.001