'Phytochip': on-chip detection of phytopathogenic RNA viruses by a new surface plasmon resonance platform.

@article{Florschtz2013PhytochipOD,
  title={'Phytochip': on-chip detection of phytopathogenic RNA viruses by a new surface plasmon resonance platform.},
  author={Kristina Florsch{\"u}tz and Anja Schr{\"o}ter and Stefan Schmieder and Wanxin Chen and Patrick Schweizer and Frank Sonntag and Norbert Danz and Keith H R Baronian and Gotthard Kunze},
  journal={Journal of virological methods},
  year={2013},
  volume={189 1},
  pages={80-6}
}
The surface plasmon resonance (SPR) based 'Phytochip' was developed to distinguish virus-infected plants from non-infected plants. The system detects DNA-RNA hybridization to show the presence of phytopathogenic viruses such as the RNA virus barley stripe mosaic virus (BSMV) in wheat leaves. To achieve this BSMV and wheat specific oligonucleotides, and a negative control yeast oligonucleotide, were immobilized on a SPR gold surface chip. After optimization of the hybridization parameters with… CONTINUE READING