'Knobs-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization.

  title={'Knobs-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization.},
  author={John B. B. Ridgway and Leonard G. Presta and Paul H Carter},
  journal={Protein engineering},
  volume={9 7},
'Knobs-into-holes' was originally proposed by Crick in 1952 as a model for the packing of amino acid side chains between adjacent alpha-helices. 'Knobs-into-holes' is demonstrated here as a novel and effective design strategy for engineering antibody heavy chain homodimers for heterodimerization. In this approach a 'knob' variant was first obtained by replacement of a small amino acid with a larger one in the CH3 domain of a CD4-IgG immunoadhesin: T366Y. The knob was designed to insert into a… CONTINUE READING

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-10 of 152 extracted citations

Monovalent antibody design and mechanism of action of onartuzumab , a MET antagonist with anti-tumor activity as a therapeutic agent

Mark Merchanta, Xiaolei Mab, +26 authors Daniel G. Yansurad
View 3 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…