'Knobs-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization.

@article{Ridgway1996KnobsintoholesEO,
  title={'Knobs-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization.},
  author={John B. B. Ridgway and Leonard G. Presta and Paul H Carter},
  journal={Protein engineering},
  year={1996},
  volume={9 7},
  pages={617-21}
}
'Knobs-into-holes' was originally proposed by Crick in 1952 as a model for the packing of amino acid side chains between adjacent alpha-helices. 'Knobs-into-holes' is demonstrated here as a novel and effective design strategy for engineering antibody heavy chain homodimers for heterodimerization. In this approach a 'knob' variant was first obtained by replacement of a small amino acid with a larger one in the CH3 domain of a CD4-IgG immunoadhesin: T366Y. The knob was designed to insert into a… CONTINUE READING

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