'GM-gene-deletor': fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants.

@article{Luo2007GMgenedeletorFL,
  title={'GM-gene-deletor': fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants.},
  author={Keming Luo and Hui Nan Duan and Degang Zhao and Xuelian Zheng and Wei Deng and Yongqin Chen and C. Neal Stewart and Richard M. McAvoy and Xiangning Jiang and Yanhong Wu and Aigong He and Yan Pei and Yi Li},
  journal={Plant biotechnology journal},
  year={2007},
  volume={5 2},
  pages={263-274}
}
Pollen- and seed-mediated transgene flow is a concern in plant biotechnology. We report here a highly efficient 'genetically modified (GM)-gene-deletor' system to remove all functional transgenes from pollen, seed or both. With the three pollen- and/or seed-specific gene promoters tested, the phage CRE/loxP or yeast FLP/FRT system alone was inefficient in excising transgenes from tobacco pollen and/or seed, with no transgenic event having 100% efficiency. When loxP-FRT fusion sequences were… CONTINUE READING
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