'Candidatus Liberibacter americanus', associated with citrus huanglongbing (greening disease) in São Paulo State, Brazil.

@article{Teixeira2005CandidatusLA,
  title={'Candidatus Liberibacter americanus', associated with citrus huanglongbing (greening disease) in S{\~a}o Paulo State, Brazil.},
  author={Diva do Carmo Teixeira and Colette Saillard and Sandrine Eveillard and Jean Luc Danet and Paulo In{\'a}cio da Costa and Antonio Juliano Ayres and Joseph M. Bov{\'e}},
  journal={International journal of systematic and evolutionary microbiology},
  year={2005},
  volume={55 Pt 5},
  pages={
          1857-62
        }
}
Symptoms of huanglongbing (HLB) were reported in São Paulo State (SPS), Brazil, in March 2004. In Asia, HLB is caused by 'Candidatus Liberibacter asiaticus' and in Africa by 'Candidatus Liberibacter africanus'. Detection of the liberibacters is based on PCR amplification of their 16S rRNA gene with specific primers. Leaves with blotchy mottle symptoms characteristic of HLB were sampled in several farms of SPS and tested for the presence of liberibacters. 'Ca. L. asiaticus' was detected in a… 

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Co-cultivation of 'Candidatus Liberibacter asiaticus' with Actinobacteria from Citrus with Huanglongbing.

Huanglongbing (HLB), also known as citrus greening disease, is a devastating disease of citrus caused by phloem-limited bacteria that have not been grown in culture, suggesting that the bacteria might be mutually beneficial to each other in culture.

Molecular detection, identification, and sequence analysis of ‘Candidatus Liberibacter asiaticus’ associated with Huanglongbing disease of citrus in North India

The study demonstrated that all examined five HLB isolates belonged to ‘CLas’ group, however, these isolates showed distinct sequence variability in three out of four genomic regions.

Liberibacters Associated with Citrus Huanglongbing in Brazil: 'Candidatus Liberibacter asiaticus' Is Heat Tolerant, 'Ca. L. americanus' Is Heat Sensitive.

The influence of temperature on sweet orange trees infected with ' Ca. L. asiaticus' or 'Ca. americanus' was studied in temperature-controlled growth chambers and the uneven distribution of these two liberibacters in São Paulo State might be in relation with these results.

Three novel lineages of 'Candidatus Liberibacter africanus' associated with native rutaceous hosts of Trioza erytreae in South Africa.

While native citrus relatives were shown to be infected with Laf-related liberibacters, nucleotide sequence data suggest that these are not alternative sources of Laf to citrus orchards, per se.

Quantitative distribution of 'Candidatus Liberibacter asiaticus' in citrus plants with citrus huanglongbing.

A quantitative polymerase chain reaction assay is used to systematically quantify the distribution of 'Ca. L. asiaticus' genomes in tissues of six species of citrus either identified in the field during survey efforts in Florida or propagated in a greenhouse in Beltsville, MD.

Confirmation of the sequence of 'Candidatus Liberibacter asiaticus' and assessment of microbial diversity in Huanglongbing-infected citrus phloem using a metagenomic approach.

Phloem metagenomic DNA provided a PCR-independent means of verifying the presence of 'Ca L. asiaticus' in infected tissue and strongly suggests that no other disease agent was present in phloem.

Quantification of Live 'Candidatus Liberibacter asiaticus' Populations Using Real-Time PCR and Propidium Monoazide.

This rapid qPCR method provides a more accurate way to determine GLB in HLB hosts which, in turn, should benefit disease epidemiology studies and serve as a crucial component in H LB management.

rRNA operons and genome size of 'Candidatus Liberibacter americanus', a bacterium associated with citrus huanglongbing in Brazil.

The results suggest that there are three ribosomal operons in a circular genome of Lam, the first liberibacter species for which such data are available.

Candidatus Liberibacter americanus sp . nov . , is Associated with Citrus Huanglongbing ( Greening Disease ) in São Paulo State , Brazil *

Candidatus Liberibacter africanus and Ca. L. asiaticus are α Proteobacteria , responsible for citrus huanglongbing (HLB) in Africa and Asia, respectively. Until 2004, the disease had never been
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References

SHOWING 1-10 OF 26 REFERENCES

Citrus huanglongbing in São Paulo State, Brazil: PCR detection of the 'Candidatus' Liberibacter species associated with the disease.

First Report of a Huanglongbing-Like Disease of Citrus in Sao Paulo State, Brazil and Association of a New Liberibacter Species, "Candidatus Liberibacter americanus", with the Disease.

The new bacterium is a liberibacter and is sufficiently different phylogenetically from known liberibacters to warrant a new species, "Candidatus Liberibacter americanus".

First Report of the Causal Agent of Huanglongbing ("Candidatus Liberibacter asiaticus") in Brazil.

The presence of "Ca. L. asiaticus" in Brazil is confirmed for the first time, which relates to the potential damage that this pathogen could cause to the citrus industry in the largest citrus-producing country in the world.

Distribution of the Huanglongbing (Greening) Liberobacter Species in Fifteen African and Asian Countries

The distribution of the two Huanglongbing (greening) liberobacter species, Liber- obacter asiaticum and Liberobacter africanum, was studied by DNA-DNA hybridization with probe In and by PCR followed by XbaI restriction of the amplified DNA.

The phloem-limited bacterium of greening disease of citrus is a member of the alpha subdivision of the Proteobacteria.

Comparisons with sequences of 16S rDNAs obtained from the GenBank data base revealed that the two citrus greening disease BLOs belong to the alpha subdivision of the class Proteobacteria, and it is proposed that this new group of bacteria is called "liberobacter".

PCR detection of the two 'Candidatus' Liberobacter species associated with greening disease of citrus.

A PCR method to detect the two liberobacter species in citrus trees by amplification of a 1160 bp fragment of their 16S rDNA is described.

Phylogenetic characterization of the bacterium-like organism associated with marginal chlorosis of strawberry and proposition of a Candidatus taxon for the organism, 'Candidatus phlomobacter fragariae'.

A Candidatus taxon is proposed for the marginal-chlorosis-associated bacterium, 'Candidatus Phlomobacter fragariae', which is a new bacterium within group 3 of the gamma subclass of Proteobacteria, a group of Gram-negative bacteria including, in particular, insect symbionts or parasites as well as enterobacteria.

Comparison of the 16S/23S ribosomal intergenic regions of "Candidatus Liberobacter asiaticum" and "Candidatus Liberobacter africanum," the two species associated with citrus huanglongbing (greening) disease.

16S/23S intergenic spacer regions from the rRNA operons of two strains of "Candidatus Liberobacter asiaticum" and one strain of "Candidatus Liberobacter africanum" were cloned and sequenced. The

Aetiology of citrus greening disease.

16S ribosomal DNA amplification for phylogenetic study

By use of this method, the phylogenetic study of extremely fastidious or highly pathogenic bacterial species can be carried out without the need to culture them.