"Wired" enzyme electrodes for amperometric determination of glucose or lactate in the presence of interfering substances.

@article{Ohara1994WiredEE,
  title={"Wired" enzyme electrodes for amperometric determination of glucose or lactate in the presence of interfering substances.},
  author={Tsutomu Ohara and Raghavan Rajagopalan and Adam Heller},
  journal={Analytical chemistry},
  year={1994},
  volume={66 15},
  pages={
          2451-7
        }
}
Glucose oxidase (GOX) or lactate oxidase (LOX) were immobilized in an osmium-based three-dimensional redox hydrogel that electrically connected the enzyme's redox centers to electrodes. The enzyme "wiring" hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed with Os-(4,4'-dimethylbpy)2Cl (termed PVI15-dmeOs) with poly(ethylene glycol) diglycidyl ether (peg). Glucose and lactate sensors exhibited typical limiting current densities of 250 and 500 microA/cm2, respectively… CONTINUE READING

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