"Spoligoriftyping," a dual-priming-oligonucleotide-based direct-hybridization assay for tuberculosis control with a multianalyte microbead-based hybridization system.

@article{Gomgnimbou2012SpoligoriftypingAD,
  title={"Spoligoriftyping," a dual-priming-oligonucleotide-based direct-hybridization assay for tuberculosis control with a multianalyte microbead-based hybridization system.},
  author={Michel Kir{\'e}opori Gomgnimbou and Edgar Abad{\'i}a and Jian Zhang and Guislaine Refregier and Stefan Panaiotov and Elizabeta Bachiyska and Christophe Sola},
  journal={Journal of clinical microbiology},
  year={2012},
  volume={50 10},
  pages={
          3172-9
        }
}
We developed "spoligoriftyping," a 53-plex assay based on two preexisting methods, the spoligotyping and "rifoligotyping" assays, by combining them into a single assay. Spoligoriftyping allows simultaneous spoligotyping (i.e., clustered regularly interspaced short palindromic repeat [CRISPR]-based genotyping) and characterization of the main rifampin drug resistance mutations on the rpoB hot spot region in a few hours. This test partly uses the dual-priming-oligonucleotide (DPO) principle… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 14 CITATIONS

Multi-drug resistant Mycobacterium tuberculosis complex genetic diversity and clues on recent transmission in Punjab, Pakistan.

  • Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • 2014
VIEW 13 EXCERPTS
CITES BACKGROUND & METHODS