"Perfect" markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat

  title={"Perfect" markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat},
  author={M. Ellis and W. Spielmeyer and K. Gale and G. Rebetzke and R. Richards},
  journal={Theoretical and Applied Genetics},
Abstract.PCR-based markers were developed to detect the point mutations responsible for the two major semi-dwarfing genes Rht-B1b (Rht1) and Rht-D1b (Rht2) in wheat. These markers were validated by testing 19 wheat varieties of known Rht genotype. They included Rht-B1b and Rht-D1b dwarfs, double-mutant varieties and tall wheats. These were correctly genotyped with the Rht-B1b and Rht-D1b-specific primers, as well as markers specific for the tall alleles Rht-B1a and Rht-D1a. Using a family of… Expand

Figures, Tables, and Topics from this paper

Distribution of dwarfing genes (Rht-B1b and Rht-D1b) in Martonvásár wheat breeding materials
The analysis of the development date of the genotypes revealed that the introduction of the dwarfing genes into Martonvasar breeding programmes started in the early 1970s, and they were widely utilized from the 1980s. Expand
Characterization of Lr34/Yr18, Rht-B1b, Rht-D1b genes in CIMMYT wheat cultivars and advanced lines using STS markers
Objective) Characterization of genes Lr34/Yr18, Rht-B1b (Rht1) and Rht-D1b (Rht2) in 263 CIMMYT wheat cultivars and advanced lines will benefit the improvement of rust resistance and plant height inExpand
Semi-dwarfing Rht-B1 and Rht-D1 loci of wheat differ significantly in their influence on resistance to Fusarium head blight
The study demonstrates that the choice of semi-dwarfing gene used in plant breeding programmes may be a significant consideration where resistance to FHB is an important breeding target. Expand
Distribution of the Rht-B1b, Rht-D1b and Rht8 reduced height genes in autumn-sown Chinese wheats detected by molecular markers
This study shows that Rht-B1b and Rht -D1b can be used in the post-anthesis heat stressed environments and were successfully used in wheat production in Chinese environments. Expand
Dwarfing genes in Spring wheat : an agronomic comparison of Rht-B1, Rht-D1, and Rht8
It is suggested that RHT-B1b and Rht-D1b are superior, in terms of yield potential, to Rht8, however, additional evaluation of RHT8 in differing environments is needed to elucidate its precise effects on yield and other agronomic traits. Expand
Rht-B 1 b and Rht-D 1 b analysis of Semidwarf wheat cv . Uruq produced by Induced Mutation
there are many genes associated with dwarfing growth in bread wheat. Two genes in particularRht-B1b and Rht-D1b were used for identification of dwarfism in many wheat varieties. In this study PCRExpand
Haplotype dictionary for the Rht-1 loci in wheat
In insights into the genetic diversity present at the Rht-1 loci in modern bread wheat and in relation to ancestral wheat accessions, novel polymorphisms, relative to 'Chinese Spring' are discovered. Expand
Isolation of the dwarfing Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker
It is shown that Rht-B1p reduced plant height by approximately 30 and 50 % in bread wheat and durum wheat, respectively, under greenhouse conditions, and was identical to the earlier identified gene Rht17. Expand
The effects of Rht semi-dwarfing alleles on agronomic traits in Korean wheat cultivars
Significant differences were recorded in culm and spike length regardless of allelic variation of these semi-dwarf genes. Expand
Novel natural allelic variations at the Rht-1 loci in wheat.
Rich genetic diversity at the Rht-1 loci is suggested and new resources for wheat breeding are provided by analysis of natural allelic variations of three homoeologous loci in Chinese wheat. Expand


Comparative molecular mapping of GA insensitive Rht loci on chromosomes 4B and 4D of common wheat (Triticum aestivum L.)
The homoeologous relationships between the GA-insensitive dwarfing genes within the Triticeae are discussed and Rht-D1c was found to be closely linked to the distally located markers Xpsr921 and Xmwg634. Expand
The effect of dwarfing genes in spring wheat in dry environments, 1. Agronomic characteristics
Near-isogenic lines of wheat were used to investigate the possible yield penalty of dwarfing genes in rainfed environments and their effect on agronomic characteristics varied depending on the genetic background but general trends were evident. Expand
Establishment and characterisation of wheat genetic mapping populations
These populations provide powerful tools for extensive mapping studies to determine the genetic factors controlling grain quality traits and other wheat characters of interest and are recognised in the models used. Expand
Breeding long coleoptile, reduced height wheats
It is suggested that GA-sensitive Rht genes could be used to select shorter height, longer-coleoptile wheats with improved establishment and seedling vigour in semidwarf varieties. Expand
‘Green revolution’ genes encode mutant gibberellin response modulators
It is shown that Rht-B1/Rht-D1 and maize dwarf-8 (d8), are orthologues of the Arabidopsis Gibberellin Insensitive (GAI) gene, which encode proteins that resemble nuclear transcription factors and contain an SH2-like domain, indicating that phosphotyrosine may participate in gibberelli signalling. Expand
A classification of the NORIN 10 and Tom Thumb dwarfing genes in British, Mexican, Indian and other hexaploid bread wheat varieties
These genes may be identified and classified by assessing the associated character of GA-insensitivity in the progeny from test crosses in the CIMMYT, Mexican, PBI, Cambridge and Indian breeding programmes and for a number of other international varieties. Expand
Permutation tests for multiple loci affecting a quantitative character.
Two extensions of the permutation-based method for estimating empirical threshold values are presented, which yield critical values that can be used to construct tests for the presence of minor QTL effects while accounting for effects of known major QTL. Expand
A rapid method for early generation selection of dwarf genotypes in wheat
A method which exploits the gibberellin insensitive reaction of lines carrying the genes combined with a ‘single seed descent’ technique is described in which most unwanted genotypes may be removed as F2 seedlings. Expand
Yield potential progress in short bread wheats in Northwest Mexico
Germplasm from the spring wheat (Trificum oesfivum L.) breeding program at the International Center for Improvement of Maize and Wheat (CIMMYT has had a major impact on the yield of irrigated springExpand
Precision mapping of quantitative trait loci.
  • Z. Zeng
  • Biology, Medicine
  • Genetics
  • 1994
A new method of QTL mapping is proposed and analyzed in this paper by combining interval mapping with multiple regression, an interval test in which the test statistic on a marker interval is made to be unaffected by QTLs located outside a defined interval. Expand