"Pathotyping" Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence.

  title={"Pathotyping" Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence.},
  author={Kate J Howell and Lucy Anne Weinert and Sarah E. Peters and Jinhong Wang and J. Fernando Hernandez-Garcia and Roy R. Chaudhuri and S. Luan and {\O}ystein Angen and V Aragon and Susanna M. Williamson and Paul R Langford and Andrew N. Rycroft and Brendan W. Wren and Duncan J Maskell and Alexander W. Tucker},
  journal={Journal of clinical microbiology},
  volume={55 9},
Haemophilus parasuis is a diverse bacterial species that is found in the upper respiratory tracts of pigs and can also cause Glässer's disease and pneumonia. A previous pangenome study of H. parasuis identified 48 genes that were associated with clinical disease. Here, we describe the development of a generalized linear model (termed a pathotyping model) to predict the potential virulence of isolates of H. parasuis based on a subset of 10 genes from the pangenome. A multiplex PCR (mPCR) was… CONTINUE READING
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Publications referenced by this paper.

A molecular serotyping multiplex PCR for Haemophilus parasuis

  • KJ Howell, J Hernandez-Garcia, +11 authors AW. Tucker
  • J Clin Microbiol 53:3812–3821
  • 2015
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