"PERFEXT": a direct method for quantitative assessment of cytokine production in vivo at the local level.

@article{Villavedra1997PERFEXTAD,
  title={"PERFEXT": a direct method for quantitative assessment of cytokine production in vivo at the local level.},
  author={M Villavedra and H Carol and Martin Hjulstr{\"o}m and Jan Holmgren and C{\'e}cil Czerkinsky},
  journal={Research in immunology},
  year={1997},
  volume={148 4},
  pages={257-66}
}
A method termed "PERFEXT", based on sequential perfusion and detergent extraction of lymphoid and non-lymphoid organs, has been developed for the quantitative measurement of cytokines produced at a local level in a given tissue. In vivo treatment of mice with Staphylococcus enterotoxin B (SEB) or lipopolysaccharide (LPS) served as the model systems. Interleukin-2 (IL2) and interferon-gamma (IFN gamma) levels were monitored by ELISA analysis of extracted samples. After local footpad (FP… CONTINUE READING