"Break copy" duplication: a model for chromosome fragment formation in Saccharomyces cerevisiae.

@article{Morrow1997BreakCD,
  title={"Break copy" duplication: a model for chromosome fragment formation in Saccharomyces cerevisiae.},
  author={Dana Morrow and Carla J. Connelly and Phil Hieter},
  journal={Genetics},
  year={1997},
  volume={147 2},
  pages={
          371-82
        }
}
Introduction of a chromosome fragmentation vector (CFV) into the budding yeast Saccharomyces cerevisiae results in a targeted homologous recombination event that yields an independently segregating chromosome fragment (CF) and an alteration in the strain's karyotype. Fragmentation with an acentric CFV directed in a centromere-proximal orientation generates a CF that contains all sequences proximal to the targeting segment and results in loss of the endogenous targeted chromosome to yield a 2N-1… CONTINUE READING

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