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水稻 Osε-cop1 基因的原核表达及多克隆抗体制备

e-COP 蛋白是真核生物分泌途径中 COPⅠ有被小泡的一个亚基。本研究利用 PCR 技术扩增水稻 e- cop 基因( Os e- cop1 )的 ORF(开放阅读框)，并克隆到原核表达载体 pET-23d 上，将表达载体 pET-Ose-cop1 转入大肠杆菌 BL21 (DE3),以 1.0 mmol/L 的 IPTG (isopropyl…
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