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A high-performance liquid chromatographic method was established for the fractionation of oxidized choline glycerophospholipids (CGPs) that contain aldehyde residues, after their derivatization with a fluorescent reagent 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H). DBD-H efficiently reacted with the aldehyde residues of(More)
The formation of phospholipid hydroperoxides was monitored in human red blood cell (RBC) membranes that had been peroxidized with an azo initiator. Peroxidation of RBC membranes caused a profound decrease in the amount of polyunsaturated fatty acids and concomitantly hydroperoxides, as primary products of peroxidation, appeared in the phospholipids.(More)
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