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Previous studies have shown that TaNHX2 transgenic alfalfa (Medicago sativa L.) accumulated more K+ and less Na+ in leaves than did the wild-type plants. To investigate whether the increased K+ accumulation in transgenic plants is attributed to TaNHX2 gene expression and whether the compartmentalization of Na+ into vacuoles or the intracellular(More)
Gene BADH was transformed into alfalfa (Medicago sativa L.) through Agrobacterium-mediated transformation method, and salt tolerance of the transgenic progenies was assessed. Among 247 plants obtained after transformation, 43 were positive by polymerase chain reaction (PCR) detection using the primers specific for BADH gene. Reverse transcription polymerase(More)
Interleukin 7/Interleukin 7 receptor (IL-7/IL-7R) signaling induces the upregulation of cyclin D1 to promote cell proliferation in lung cancer, but its role in preventing the apoptosis of non-small cell lung cancer (NSCLC) cell lines remains unknown. To study the role of IL-7 in lung cancer cell apoptosis, normal HBE cells as well as A549 and H1299 NSCLC(More)
Soybean is a difficult crop to manipulate through Agrobacterium tumefaciens-mediated genetic transformation. Plant–bacterium interaction plays an important role in the transformation process. Being rich with isoflavones, soybean may have an adverse effect on the A. tumefaciens-mediated genetic transformation. To investigate whether high content of(More)
Maize cultivar zhengdan958 was selected as materials. The sub-cellular distribution of soluble calcium at different phases was shown by the potassium-pyroantinonate-precipitation method and transmission electron microscopy. The results showed that the deposits of calcium antimonate as the indicator for Ca(2+) localization were mainly concentrated within the(More)
Antagonists and sonication treatment relieved the structural barriers of Agrobacterium entering into cells; hindered signal perception and transmission; alleviated defense responses and increased cell susceptibility to Agrobacterium infection. Soybean gene expression analysis was performed to elucidate the general response of soybean plant to Agrobacterium(More)
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