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Methods based on enzyme labels have been developed for colorimetric immunoassays, but most involve poor sensitivity and are unsuitable for routine use. Herein, we design an enhanced colorimetric immunoassay for prostate-specific antigen (PSA) coupling with an enzyme-cascade-amplification strategy (ECAS-CIA). In the presence of target PSA, the labeled… (More)
A label-free, non-enzyme immunosensing strategy is designed for ultrasensitive electronic detection of disease-related proteins (carcinoembryonic antigen as a model) by using gold nanoparticle-based bio-bar codes and an in situ amplified DNA-based hybridization chain reaction.
Individual standard stock samples of brevetoxin B (PbTx-2) and aflatoxin B 1 (AFB 1) were purchased from Express Technol. Co. Ltd. (Beijing, China). Monoclonal mouse anti-PbTx-2 antibody and monoclonal mouse anti-AFB 1 antibody were provided by Dingguo Biotechnol. Co. Ltd (Beijing, China). Mouse monoclonal anti-human antibody against prostate-specific… (More)