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The phenylalanine ammonia lyase (PAL) cDNA was amplified from Petroselinum crispum RNA by using the RT-PCR technique. The amplified 2.2 kb DNA fragment was sequenced and inserted into expression vector pET23b. The resulting plasmid pET23bPAL was then transformed into E. coli JM109DE3. The expressed PAL protein in JM109DE3 (pET23bPAL) accounted for more than(More)
The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production. In our studies, we found that AtCSR was associated with cadmium (Cd)-sensitive response in Arabidopsis. Our results show that AtCSR expression was triggered by Cd-stress in(More)
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