Zhongyuan Liu

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Many heat stress transcription factors (Hsfs) and heat shock proteins (Hsps) have been identified to play important roles in the heat tolerance of plants. However, many of the key factors mediating the heat response pathways remain unknown. Here, we report that two genes, which are targets of TAS1 (trans-acting siRNA precursor 1)-derived small interfering(More)
Arabidopsis thaliana HYPONASTIC LEAVES1 (HYL1) is a microRNA (miRNA) biogenesis protein that contains two N-terminal double-stranded RNA binding domains (dsRBDs), a putative nuclear localization site (NLS), and a putative protein-protein interaction domain. The interaction of HYL1 with DICER-LIKE1 is important for the efficient and precise processing of(More)
BACKGROUND It was proposed that there are at least 250 enzymes in M. tuberculosis involved in lipid metabolism. Rv0045c was predicted to be a hydrolase by amino acid sequence similarity, although its precise biochemical characterization and function remained to be defined. METHODOLOGY/PRINCIPAL FINDINGS We expressed the Rv0045c protein to high levels in(More)
Cubic boron nitride (cBN) is a well known superhard material that has a wide range of industrial applications. Nanostructuring of cBN is an effective way to improve its hardness by virtue of the Hall-Petch effect--the tendency for hardness to increase with decreasing grain size. Polycrystalline cBN materials are often synthesized by using the martensitic(More)
A novel carbon allotrope of C-centered orthorhombic C(8) (Cco-C(8)) is predicted by using a recently developed particle-swarm optimization method on structural search. Cco-C(8) adopts a sp(3) three-dimensional bonding network that can be viewed as interconnected (2,2) carbon nanotubes through 4- and 6-member rings and is energetically more favorable than(More)
Combining the advantages of signal-on strategy and nicking endonuclease assisted electrochemistry signal amplification (NEAESA), a new sensitive and signal-on electrochemical DNA biosensor for the sequence specific DNA detection based on NEAESA has been developed for the first time. A Hairpin-shape probe (HP), containing the target DNA recognition sequence,(More)
u = 0, x ∈ ∂Ω, where p > 1, Ω ⊂ R is a bounded domain, Ω+i and Ωj are mutually disjoint subdomains of Ω and Ωi (resp. Ωj ) are characteristic functions of Ω + i (resp. Ω − j ), q is a harmonic function. We show that if Ω is a simply-connected smooth domain, then for any given C-stable critical point of Kirchhoff-Routh function W(x1 , · · · , xm, x1 , · · ·(More)
A novel Cu(2)O-SiO(2) nanostructured particle was synthesized by a solution-phase method and was adopted for construction of a label-free amperometric immunosensor. The porous Cu(2)O-SiO(2) nanoparticles had good redox electrochemical activity, large surface-to-volume ratio, film-forming ability and high stability. The physical morphology and structure of(More)
Three-dimensional N-doped hierarchically porous carbon (3D NHPC) was synthesized successfully without using any surfactant or etching agent. This simple and effective synthesis method was accomplished by solvothermal synthesis followed by pyrolysis. The physical morphology and chemical composition of 3D NHPC were verified by scanning electron microscopy,(More)
Insect antimicrobial peptides (AMPs) have a broad antimicrobial spectrum. To aid the characterization of the gene function and further applications, we cloned the gene of cecropinXJ into the prokaryotic expression vector pET32a and expressed cecropinXJ in Escherichia coli BL2l (DE3). Following induction by isopropyl-β-D-thiogalactoside (IPTG), a 25 kDa(More)