Zhong W. Liu

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The blind patch clamp whole-cell recording technique for CA1 pyramidal neurons in hippocampal slices was carefully introduced, the key procedures and the points for attention were emphasized. Using the "blind" method, we analyzed the properties of synaptic activity, the current features of Ca2+ channels and glutamate, GABA receptors. Our results provide a(More)
Using blind whole cell recording technique on rat hippocampal slices, the function and mechanisms of P/N leak subtraction of Clampex program in pClamp acquisition software were studied. The ways of picking up sub-pulse current and properly selecting parameters, including time course, polarization, sub-holding potential, numbers and location of P/N sub-pulse(More)
Tissue culture from hippocampal CA1 or dentate gyrus (DG) region was established on the basis of previous neuronal culture technique. The viability, intracellular calcium concentration and brain-derived neurotrophic factor (BDNF) mRNA expression of the two kinds of neurons after anoxia were observed in the counting in the confocal microscopic field and in(More)
Intracellular free Ca2+ concentration ([Ca2+]i) was measured by laser scanning confocal microscope, using Ca2+ indicator Fluo-3 in cultured hippocampal CA1 neurons isolated from newborn rat. The results showed that acute anoxia induced a rapid increase of [Ca2+]i in hippocampal CA1 neurons, and this increase could be attenuated by 100 mumol/L adenosine(More)
The effects of recombinant human interleukin-1 beta (rhIL-1 beta) and interleukin-2 (rhIL-2) on the cultured rat hippocampal neurons were investigated with intracellular recording and extracellular micropressure ejection techniques. Experimental results showed that all of the neurons tested with rhIL-1 beta (100 U/ml) exhibited a 4.20 +/- 1.86 mV membrane(More)
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