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Single chain variable fragments (scFvs) against diethylstilbestrol (DES) were selected from the splenocytes of non-immunized mice by ribosome display technology. A naive library was constructed and engineered to allow in vitro transcription and translation using an E. coli lysate system. Alternating selection in solution and immobilization in microtiter(More)
Fast detection is important in screening large-scale samples. This study establishes a direct competitive ELISA method (dcTELISA) based on an enzyme thermistor for fast atrazine (ATZ) detection. ATZ competes with β-lactamase-labeled ATZ (ATZ-E) for the binding sites on anti-ATZ monoclonal antibody (mAb). The mAb are covalently bound to Controlled Pore Glass(More)
Six Co-Rh/Fe 3 O 4 catalysts with different cobalt loadings were prepared by the co-precipitation of RhCl 3 , Co(NO 3) 2 , and Fe(NO 3) 3 using Na 2 CO 3 as the precipitant. These catalysts were tested for dicyclopentadiene (DCPD) hydroformylation to monoformyltricyclodecenes (MFTD) and diformyltricyclodecanes (DFTD). The results showed that the MFTD(More)
A novel label-free fluorescence assay for detection of Hg2+ was developed based on the Hg2+-binding single-stranded DNA (ssDNA) and SYBR Green I (SG I). Differences from other assays, the designed rich-thymine (T) ssDNA probe without fluorescent labelling can be rapidly formed a T-Hg2+-T complex and folded into a stable hairpin structure in the presence of(More)
A new surface-initiated polymerization based on pasting the initiator on a sensor chip surface was applied to prepare a malachite green (MG) imprinted ultrathin film on a surface plasmon resonance (SPR) sensor. First, the initiator (2,2-azoisobutyronitrile) was pasted on the gold surface using polyvinyl chloride (PVC). The initiator-covered gold chip was(More)
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