Zhiming Rao

Meijuan Xu21
Taowei Yang18
Wenfang Dou6
21Meijuan Xu
18Taowei Yang
6Wenfang Dou
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The genes involved in l-arginine biosynthesis in Corynebacterium crenatum are organized as the argCJBDFRGH cluster like in Corynebacterium glutamicum. However, the argC~H cluster of the C. crenatum SYPA 5-5, which is an industrialized l-arginine producer, had a lethal mutation occurring in the ArgR repressor encoding gene. The argC~H cluster with an(More)
Acetoin, a major extracellular catabolic product of Bacillus subtilis cultured on glucose, is widely used to add flavor to food and also serves as a precursor for chemical synthesis. The biosynthesis of acetoin from pyruvate requires the enzymes α-acetolactate synthase (ALS) and α-acetolactate decarboxylase (ALDC), both of which are encoded by the alsSD(More)
BACKGROUND Previously, a safe strain, Bacillus amyloliquefaciens B10-127 was identified as an excellent candidate for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD). However, B. amyloliquefaciens fermentation yields large quantities of acetoin, lactate and succinate as by-products, and the 2,3-BD yield remains prohibitively low for(More)
Corynebacterium crenatum SYPA is an aerobic and industrial l-arginine producer. A lysE deletion mutant was constructed searching a natural function of this in C. crenatum SYPA. Tracking determination of intracellular and extracellular l-arginine in the minimum culture CGXII showed that LysE did play an important role in transporting l-arginine from(More)
N-Acetyl-l-glutamate kinase (EC is first committed in the specific l-arginine pathway of Corynebacterium sp. A limited increase of l-arginine production for the argB overexpression in the engineering C. creantum SYPA-CCB strain indicated that l-arginine feedback inhibition plays an influence on the l-arginine production. In this study, we have(More)
BACKGROUND Bacillus amyloliquefaciens B10-127 exhibited an excellent ability for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD) from biodiesel-derived glycerol. However, the accumulation of by-products (acetoin, acetoin, lactate and succinate) and the 2,3-BD yield remains prohibitively low for commercial production. RESULTS Several(More)
Acetoin reductase/2,3-butanediol dehydrogenase (AR/BDH), which catalyzes the interconversion between acetoin and 2,3-butanediol, plays an important role in distribution of the products pools. This work characterized the Bacillus subtilis AR/BDH for the first time. The enzyme showed very different pH preferences of pH 6.5 for reduction and pH 8.5 for(More)
Arginine biosynthesis in Corynebacterium glutamicum proceeds via a pathway that is controlled by arginine through feedback inhibition of NAGK, the enzyme that converts N-acetyl-l-glutamate (NAG) to N-acety-l-glutamy-l-phosphate. In this study, the gene argB encoding NAGK from C. glutamicum ATCC 13032 was site-directed, and the l-arginine-binding sites of(More)
Reactive oxygen species (ROS) is an inherent consequence to all aerobically living organisms that might lead to the cells being lethal and susceptible to oxidative stress. Bacillus pumilus is characterized by high-resistance oxidative stress that stimulated our interest to investigate the heterologous expression and characterization of heme-catalase as(More)