Share This Author
PAS domain containing chemoreceptor couples dynamic changes in metabolism with chemotaxis
- Zhihong Xie, L. Ulrich, I. Zhulin, G. Alexandre
- BiologyProceedings of the National Academy of Sciences
- 19 January 2010
A chemoreceptor is characterized in A. brasilense, which functions as a redox sensor that enables the cells to seek microaerophilic conditions that support optimum nitrogen fixation, illustrating a strategy by which cells optimize chemosensing to signaling cues that directly affect current metabolic activities and thus revealing a mechanism by which chemotaxis is coordinated with dynamic changes in cell physiology.
Drosophila TIMP is a potent inhibitor of MMPs and TACE: similarities in structure and function to TIMP-3.
The N-terminal inhibitory domain of the only TIMP produced by Drosophila (dN-TIMP) was expressed in Escherichia coli and folded in vitro and shown to have conserved most of the functions of the ancestral TIMP gene.
Synergistic Interaction between Candida albicans and Commensal Oral Streptococci in a Novel In Vitro Mucosal Model
Criteria of mucosal invasion by the biofilm microorganisms suggested that the esophageal mucosa is more permissive to invasion than the oral mucosa, suggesting the pathogenic potential of C. albicans is limited.
Reactive Site Mutations in Tissue Inhibitor of Metalloproteinase-3 Disrupt Inhibition of Matrix Metalloproteinases but Not Tumor Necrosis Factor-α-converting Enzyme*
- Shuo Wei, M. Kashiwagi, Smitha Kota, Zhihong Xie, H. Nagase, K. Brew
- BiologyJournal of Biological Chemistry
- 23 September 2005
The mutant proteins are effective inhibitors of tumor necrosis factor α release from phorbol ester-stimulated cells, indicating that they provide a lead for engineering TACE-specific inhibitors that may reduce side effects arising from MMP inhibition and are possibly useful for treatment of diseases associated with excessive TNF-α levels such as rheumatoid arthritis.
Function of a Chemotaxis-Like Signal Transduction Pathway in Modulating Motility, Cell Clumping, and Cell Length in the Alphaproteobacterium Azospirillum brasilense
- Amber Bible, Bonnie B Stephens, D. Ortega, Zhihong Xie, G. Alexandre
- BiologyJournal of bacteriology
- 18 July 2008
It is proposed that the Che1 chemotaxis-like pathway modulates the cell length as well as clumping behavior, suggesting a link between these two processes and is consistent with a model in which the function of the Che2 pathway in regulating these cellular functions directly affects flocculation.
Roles of individual enzyme-substrate interactions by alpha-1,3-galactosyltransferase in catalysis and specificity.
Mutagenesis indicates that His(280), which is projected to interact with the 2-OH of the galactose moiety of UDP-Gal, is a key residue in the stringent donor substrate specificity through its role in stabilizing the bound UDP- Gal in a suitable conformation for catalysis.
A quantitative real-time RT-PCR assay for mature C. albicans biofilms
The real-time qRT-PCR assay is more accurate than the XTT assay when measuring small-medium size effects of anti-fungal agents against mature biofilms, and is also more appropriate when mature biofilm susceptibility to anti-Fungal Agents is tested on complex biological surfaces, such as organotypic cultures.
Abundance and Diversity of Soybean-Nodulating Rhizobia in Black Soil Are Impacted by Land Use and Crop Management
- Jun Yan, Xiao-zeng Han, Wen Feng Chen
- Medicine, BiologyApplied and Environmental Microbiology
- 20 June 2014
The results revealed that different land uses and crop management could not only alter the diversity and abundance of soybean rhizobia, but also change interactions between Rhizobia and legume or nonlegume plants, which offered novel information about the biogeography of rhizobial communities.
Role of Bcr1-Activated Genes Hwp1 and Hyr1 in Candida Albicans Oral Mucosal Biofilms and Neutrophil Evasion
It is found that a bcr1/bcr1 mutant did not form significant biofilm on the tongues of immunocompromised mice, and a hyr1/hyr1 mutant had significantly attenuated virulence in the mouse oral biofilm model of infection.