Zhifeng Song

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β-N-Acetylglucosaminidases serve important biological functions and various industrial applications. A glycoside hydrolase family 3 β-N-acetylglucosaminidase gene was cloned from Sphingobacterium sp. HWLB1 and expressed in Escherichia coli BL21 (DE3). The purified recombinant enzyme (rNag3HWLB1) showed apparent optimal activity at pH 7.0 and 40 °C. In the(More)
A glycoside hydrolase family 5 β-mannanase-encoding gene was cloned from Bacillus sp. HJ14 isolated from saline soil in Heijing town. Coding sequence of mature protein (without the predicted signal peptide from M1 to A30) was successfully expressed in Escherichia coli BL21 (DE3). Purified recombinant mannanase (rMan5HJ14) exhibited optimal activity at pH(More)
The operating status of equipment are closely related to the operation conditions of hydroelectric generating unit. A critical issue of maintenance automation system is how to make performance evaluation and states analysis at the same operation conditions about the hydroelectric generating equipment. A condition monitoring and performance evaluation method(More)
A new coil unwinding roller operating framework of leveling line in hot rolled flat sheet metal factory is brought out based on analyzed the shortcomings of the original operating system. The main features of the new system and the design thoughts of its hydraulic operation system are described in detail. A open controlling system is used which is based on(More)
A programmable automation based on data medium Moby-I is designed in this paper. The whole scheme of the system is brought forward firstly. And then, the data structures of the data medium Moby-I is programmed, the sequence of the multistation assembly control are expatiated, which are focused on the data exchange of general mechanism between PLC system and(More)
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