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BACKGROUND & OBJECTIVES Diagnosis for Mycoplasma pneumoniae usually relies on serological tests. PCR technology has some advantages but also limitations. The optimal selection for these tests still needs discussion. This paper reviews the overall diagnostic accuracy of PCR versus serological assays for diagnosis of M. pneumoniae infections and to identify(More)
OBJECTIVE To investigate the local epidemiology of Klebsiella penumoniae carrying bla(CTX-M-15) in southern China and to characterize the genetic environment of bla(CTX-M-15). METHODS PCR and DNA sequencing were used to detect and characterize the genetic contexts of bla(CTX-M-15). The clonal relatedness of isolates carrying bla(CTX-M-15) was determined(More)
OBJECTIVES To study morphological feature, biochemical characteristic and antibiotic resistance of Robinsoniella peoriensis (R.peoriensis) strain. METHODS The clinical R.peoriensis strain was isolated from ananus swab samples being screened in ICU. Biochemical characteristic of the strain was completed by fully automatic microbial identification and drug(More)
A 139,622-bp IncI1 ST71 conjugative plasmid pEC012 from an avian Escherichia coli D-ST117 strain was sequenced, which carried five IS26-bracketed resistance modules: IS26-fosA3-orf1-orf2-Δorf3-IS26, IS26-fip-ΔISEcp1-bla CTX-M-65-IS903D-iroN-IS26, IS26-ΔtnpR-bla TEM-1-rmtB-IS26, IS26-oqxAB-IS26, and IS26-floR-aac(3)-IV-IS26. The backbone of pEC012 was(More)
OBJECTIVE To investigate the clonal relatedness of local bla(OXA-58)-carrying Acinetobacter baumannii clinical isolates. METHODS Non-duplicated isolates of Acinetobacter baumannii were collected in West China Hospital and verified by recA sequencing. Acquired bla(OXA-58) gene and natural bla(OXA51/66) genes were detected by PCR. Strain typing for(More)
OBJECTIVE To investigate the incidence and clinical features of non-Clostridium difficile (C. difficile) associated nosocomial diarrhea in intensive care unit (ICU) caused by Klebsiella oxytoca and Clostridium perfringens. METHODS The faeces of 102 patients with non-C. difficile associated nosocomial diarrhea in ICU of West China Hospital, were collected(More)
OBJECTIVE To investigate the prevalence of genes encoding 16S rRNA methylase and the spreading path of these genes in 374 clinical isolate of enterobacteriaceae. METHODS The genes encoding 16S rRNA methylase in 374 clinical isolate of enterobacteriaceae was detected with PCR. The sequence homogeny analyses were carried out with the NCBL BLAST program and(More)
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