Zhi-wei Yang

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AIM To study the immune response in mice induced by DNA vaccine encoding immunogenic protein of Lgeionella pneumophila (LP) and to examine its protective efficacy against LP in mice. METHODS The eukaryotic expression recombinant plasmid of LP immunogenic protein gene (pcDNA3.1-ip) was constructed and used to immunize BALB/c female mice intramuscularly,(More)
We present a case of a patient with rare anatomy of a maxillary second molar with three mesiobuccal root canals and a maxillary third molar with four separate roots, identified using multi‐slice computed topography (CT) and three‐dimensional reconstruction techniques. The described case enriched/might enrich our knowledge about possible anatomical(More)
AIM To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani and detect expression of the gene in NIH3T3 cells. METHODS Amastin gene was amplified from nuclear DNA of Leishmania Donovani isolates and cloned into an eukaryotic expression vector pcDNA3.1(+). The recombinant plasmid was named pcDNA3.1-amastin. NIH3T3 cell(More)
AIM To study the effect of the hTR-siRNA adenovirus on hTR mRNA gene silence, telomerase activity inhibition and anti-tumor in vitro. METHODS RNAi adenovirus vector, Ad-hTR-siRNA, and negative control Ad-NT-siRNA were constructed by an improved ligation method. Different tumor cells and liver cell line, HL-7702, were infected with 100 MOI of the(More)
AIM To detect the concentration of soluble FcγRIIb in blood sera of SLE patients and healthy controls, then to obtain recombinant human soluble FcγRIIb protein (husFcγRIIb) in Escherichia coli (E.coli) and examine its binding capability with immune complexes (IC) and its effect on IgM secretion by B cells. METHODS The concentration of husFcγRIIb in blood(More)
In order to assess the protective effects of the DNA vaccine (pcDip/pilE) against Legionella pneumophila, the coding sequences of the 2 proteins were cloned into the pET32a(+) and pcDNA3.1(+) vectors. To provide an enhanced immunological response, the proteins were linked together. In this study, the A/J mouse model was used for examine the immunogenicity(More)
OBJECTIVE To clone and express the avastin gene of two Leishmania donovani isolates from Sichuan Province of China. METHODS Avastin gene was amplified from nuclear DNA of two L.donovani isolates, cloned into pcDNA3.1(+), and sequenced by the dideoxy chain termination. NIH3T3 cell was transfected by recombinant plasmid. Transient expression of avastin gene(More)
OBJECTIVE To construct a recombinant plasmid containing Lgeionella pneumophila pilE gene, detect its expression in NIH3T3 cells and evaluate its immunogenicity. METHODS PilE gene (LP) was amplified from Legionella pneumophila DNA by PCR and inserted into pcDNA3.1(+) vector to construct the recombinant plasmid pcDNA3.1-pilE, which as verified by(More)
OBJECTIVE To investigate the immunogenicity of recombinant plasmid pcDNA3.1-amastigote with Leishmania donovani amastigote gene. METHODS Eighteen female BALB/c mice were randomly divided into experimental group and control group. Mice in experimental group and control group were intramuscularly injected with 50 microg recombinant plasmid(More)
OBJECTIVE To construct the recombinant plasmid of Legionella pneumophila immunogenic protein gene (ip) and detect the immunoprotein expression in NIH3T3 cells. METHODS The immunogenic protein gene was amplified from DNA of Legionella pneumophila by polymerase chain reaction (PCR), then inserted into pcDNA3. 1 (+) vector. The recombinant plasmid was named(More)
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